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Document 3221
DOCN M94A3221
TI More apoptosis of T-cell subsets were observed in the more advanced
HIV-infected individuals using BM-1/JIMRO mAb and flow cytometric
analysis.
DT 9412
AU Ruxrungtham K; Kashiwagi N; Adachi M; Sirivichayakul S; Maruyama I;
Phanuphak P; Chulalongkom Hospital, Bangkok, Thailand.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):125 (abstract no. PA0119). Unique
Identifier : AIDSLINE ICA10/94369354
AB BACKGROUND: Apoptosis is one of the proposed mechanisms of the depletion
of CD4+ T-cells in AIDS. BM-1/JIMRO mAb recognizes Le(Y) carbohydrate
antigen which is closely correlated with the process of apoptosis.
OBJECTIVE: To analyse the expression of an apoptotic marker-BM-1 (Le(Y))
Ag on peripheral blood T-cell subsets from HIV-infected individuals with
different stages of the disease. METHODS: Heparinized blood was
collected from 60 patients (12 Asymp, 7 PGL, 25 ARC, 16 AIDS) and 20 HIV
seronegative individuals. 93% of the patients were sexually-acquired
(87% Heterosexual) and 7% IVDU. Sex: M/F = 48/12. PBMC's were prepared
by Ficoll hypaque separation. Cells were then double stained with
CD4/CD8, BM-1/CD4, BM-1/CD8 and HLA-DR/CD8 and analyzed using flow
cytometry. RESULTS: CD4+ T cells expressing BM-1 antigen was
significantly elevated, but only in the patients with AIDS (p < 0.001,
by Mann-Whitney). Interestingly, BM-1 Ag was highly expressed on CD8+ T
cells from all groups of patients (PGL, ARC and AIDS, p < 0.05, < 0.05
and < 0.001) except the asymptomatic. Whereas, HLA-DR+/CD8+ cells in all
groups including the asymptomatic were significantly higher than the
controls (p < 0.001, Mann-Whitney). DISCUSSION: In this preliminary
study, more apoptosis of T-cell subsets (both CD4+ and CD8+) were
observed in the more advanced HIV-infected patients than the controls.
All groups of patients showed significantly high level of activated-CD8+
(DR+) cells. Interestingly, in the asymptomatic patients revealed
significantly high DR+/CD8+ but not BM-1+/CD8+ cells which suggests that
BM-1 may not be an activation marker. BM-1 may be a valuable marker for
the management of AIDS and may also shed more light in HIV pathogenesis.
DE *Antibodies, Monoclonal Antigens, CD/BLOOD Antigens, CD4/BLOOD
Antigens, CD8/BLOOD *Apoptosis AIDS-Related Complex/BLOOD/IMMUNOLOGY
Biological Markers/BLOOD Cells, Cultured Comparative Study Female
Flow Cytometry/*METHODS Human HIV Infections/BLOOD/*IMMUNOLOGY HIV
Seronegativity HIV Seropositivity/BLOOD/*IMMUNOLOGY Male T-Lymphocyte
Subsets/*PATHOLOGY T4 Lymphocytes/*IMMUNOLOGY/PATHOLOGY MEETING
ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).